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2021 Vol.51, Issue 4 Preview Page

Original Article

Antiviral Effects of Mandarin Somatid Natural Gel on SARS-CoV-2 Infection in vitro
Yeonhwa Kim1
,
Eun Yong Oh1
,
Sohyun Park1
,
Sang-Myeong Lee1†
1College of Veterinary Medicine, Chungbuk National University, Cheongju 28644, Republic of Korea
*smlee@chungbuk.ac.kr
31 December 2021. pp. 200-208
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Abstract

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was reported in Wuhan, China, in December 2019 and continues to spread worldwide. Although several vaccines were developed and are being used worldwide, antivirals are still needed for people who are not vaccinated or who are vaccinated but infected due to insufficient immune responses. Plant-derived natural substances have long been studied to develop drugs for infectious diseases, and accumulated evidences support a possibility that these may well be efficacious in reducing the risk of SARS-CoV-2 infection. In the present study, it was determined whether somatid natural gels (SNGs) prepared from mandarin, ginseng, or garlic inhibit the replication of SARS-CoV-2 in vitro study. Cytopathic effect (CPE) inhibition assay, immunofluorescence assay (IFA), and quantitative real-time polymerase chain reaction (qRT-PCR) were performed to evaluate the effect of SNGs on SARS-CoV-2 replication in cell culture. Among three SNGs, the mandarin SNG had the highest inhibitory effect on SARS-CoV-2-induced CPE, while the garlic SNG showed no activity. When viral protein levels were identified by IFA, mandarin SNG treatment suppressed N protein expression in a dose-dependent manner. In addition, viral RNA copy numbers and infectious viral titers of SARS-CoV-2 were also significantly reduced by mandarin SNG. Our results suggest that mandarin SNG exerts antiviral activities against SARS-CoV-2, implying a possible use of these natural materials as a preventive or therapeutic agent for COVID19.

Keywords
SARS-CoV-2
Somatid nature gel
Mandarin
References
1

Sohrabi C, Alsafi Z, O'Neill N, Khan M, Kerwan A, Al-Jabir A, et al. World Health Organization declares global emergency: A review of the 2019 novel coronavirus (COVID-19). Int J Surg 2020;76:71-6.

10.1016/j.ijsu.2020.02.03432112977PMC7105032
2

Keogh-Brown MR, Smith RD. The economic impact of SARS: How does the reality match the predictions? Health Policy 2008;88:110-20.

10.1016/j.healthpol.2008.03.00318436332PMC7114672
3

Castells MC, Phillips EJ. Maintaining Safety with SARS-CoV-2 Vaccines. N Engl J Med 2021;384:643-9.

10.1056/NEJMra203534333378605PMC7787218
4

Sharun K, Dhama K, Patel SK, Pathak M, Tiwari R, Singh BR, et al. Ivermectin, a new candidate therapeutic against SARS-CoV-2/COVID-19. Ann Clin Microbiol Antimicrob 2020;19:23.

10.1186/s12941-020-00368-w32473642PMC7261036
5

Xiao X, Wang C, Chang D, Wang Y, Dong X, Jiao T, et al. Identification of Potent and Safe Antiviral Therapeutic Candidates Against SARS-CoV-2. Front Immunol 2020;11:586572.

10.3389/fimmu.2020.58657233324406PMC7723961
6

Park SJ, Yu KM, Kim Y Il, Kim SM, Kim EH, Kim SG, et al. Antiviral efficacies of FDA-approved drugs against SARS-COV-2 infection in ferrets. MBio 2020;11:e01114-20.

10.1128/mBio.01114-20
7

Jean SS, Lee PI, Hsueh PR. Treatment options for COVID-19: The reality and challenges. J Microbiol Immunol Infect 2020;53:436-43.

10.1016/j.jmii.2020.03.03432307245PMC7129535
8

Noreen S, Maqbool I, Madni A. Dexamethasone: Therapeutic potential, risks, and future projection during COVID-19 pandemic. Eur J Pharmacol 2021;894:173854.

10.1016/j.ejphar.2021.17385433428898PMC7836247
9

Beigel JH, Tomashek KM, Dodd LE, Mehta AK, Zingman BS, Kalil AC, et al. Remdesivir for the Treatment of Covid-19 - Final Report. N Engl J Med 2020;383:1813-26.

10.1056/NEJMoa200776432445440PMC7262788
10

Dougan M, Nirula A, Azizad M, Mocherla B, Gottlieb RL, Chen P, et al. Bamlanivimab plus Etesevimab in Mild or Moderate Covid-19. N Engl J Med 2021;385:1382-92.

10.1056/NEJMoa210268534260849PMC8314785
11

Babaei F, Mirzababaei M, Nassiri-Asl M, Hosseinzadeh H. Review of registered clinical trials for the treatment of COVID-19. Drug Dev Res 2021;82:474-93.

10.1002/ddr.2176233251593PMC7753306
12

Bhuiyan FR, Howlader S, Raihan T, Hasan M. Plants Metabolites: Possibility of Natural Therapeutics Against the COVID-19 Pandemic. Front Med 2020;7:444.

10.3389/fmed.2020.0044432850918PMC7427128
13

Mukhtar M, Arshad M, Ahmad M, Pomerantz RJ, Wigdahl B, Parveen Z. Antiviral potentials of medicinal plants. Virus Res 2008;131:111-20.

10.1016/j.virusres.2007.09.00817981353PMC7114233
14

Lin LT, Hsu WC, Lin CC. Antiviral natural products and herbal medicines. J Tradit Complement Med 2014;4:24-35.

10.4103/2225-4110.12433524872930PMC4032839
15

Islam R, Parves MR, Paul AS, Uddin N, Rahman MS, Mamun A Al, et al. A molecular modeling approach to identify effective antiviral phytochemicals against the main protease of SARS-CoV-2. J Biomol Struct Dyn 2021;39:3213-24.

10.1080/07391102.2020.1761883PMC7232885
16

Huang F, Li Y, Leung EL, Liu X, Liu K, Wang Q, et al. A review of therapeutic agents and Chinese herbal medicines against SARS-COV-2 (COVID-19). Pharmacol Res 2020;158:104929.

10.1016/j.phrs.2020.10492932442720PMC7237953
17

Joung O, Cho YS, Cho CW, Lee KA, Shim JH, Cho MC, et al. The Effects of Somatid on the Cytotoxicity of Cancer Cells and Human Papillomavirus Type 16 E6 and E7 Oncogenes. YAKHAK HOEJI 2000;44:340-6.

18

Lee JD. Natural extracts, and natural somatid gel method and a multi-functional material powder prepared using the same composition. Korean patent KR 10-1628995. 2016 Jun 9.

19

Schmidtke M, Schnittler U, Jahn B, Dahse H, Stelzner A. A rapid assay for evaluation of antiviral activity against coxsackie virus B3, influenza virus A, and herpes simplex virus type 1. J Virol Methods 2001;95:133-43.

10.1016/S0166-0934(01)00305-6
20

Amporndanai K, Meng X, Shang W, Jin Z, Rogers M, Zhao Y, et al. Inhibition mechanism of SARS-CoV-2 main protease by ebselen and its derivatives. Nat Commun 2021;12:3061.

10.1038/s41467-021-23313-734031399PMC8144557
21

Park YC, Yang YT, Kim JY, Lee CH, Kang SH, Kang JH, et al. Characteristics of Flavonoids in Juice and Cluster Analysis of Satsuma Mandarin Germplasms. Korean J Plant Resour 2015;28:16-25

10.7732/kjpr.2015.28.1.016
22

Tungmunnithum D, Thongboonyou A, Pholboon A, Yangsabai A. Flavonoids and Other Phenolic Compounds from Medicinal Plants for Pharmaceutical and Medical Aspects: An Overview. Medicines 2018;5:93.

10.3390/medicines503009330149600PMC6165118
23

Chan JFW, Yuan S, Kok KH, To KKW, Chu H, Yang J, et al. A familial cluster of pneumonia associated with the 2019 novel coronavirus indicating person-to-person transmission: a study of a family cluster. Lancet 2020;395:514-23.

10.1016/S0140-6736(20)30154-9
24

Chen N, Zhou M, Dong X, Qu J, Gong F, Han Y, et al. Epidemiological and clinical characteristics of 99 cases of 2019 novel coronavirus pneumonia in Wuhan, China: a descriptive study. Lancet 2020;395:507-13.

10.1016/S0140-6736(20)30211-7
25

Kim TY, Jeon S, Jang Y, Gotina L, Won J, Ju YH, et al. Platycodin D, a natural component of Platycodon grandiflorum, prevents both lysosome- and TMPRSS2-driven SARS-CoV-2 infection by hindering membrane fusion. Exp Mol Med 2021;53:956-72.

10.1038/s12276-021-00624-934035463PMC8143993
26

van de Sand L, Bormann M, Alt M, Schipper L, Heilingloh CS, Steinmann E, et al. Glycyrrhizin effectively inhibits sars-cov-2 replication by inhibiting the viral main protease. Viruses 2021;13:609.

10.3390/v1304060933918301PMC8066091
27

Hong S, Seo SH, Woo SJ, Kwon Y, Song M, Ha NC. Epigallocatechin Gallate Inhibits the Uridylate-Specific Endoribonuclease Nsp15 and Efficiently Neutralizes the SARS-CoV-2 Strain. J Agric Food Chem 2021;69:5948-54.

10.1021/acs.jafc.1c0205034015930PMC8146138
28

Bellavite P, Donzelli A. Hesperidin and SARS-CoV-2: New light on the healthy function of citrus fruits. Antioxidants 2020;9:742.

10.3390/antiox908074232823497PMC7465267
29

Dong W, Wei X, Zhang F, Hao J, Huang F, Zhang C, et al. A dual character of flavonoids in influenza A virus replication and spread through modulating cell-autonomous immunity by MAPK signaling pathways. Sci Rep 2014;4:7237.

10.1038/srep0723725429875PMC4246350
30

Haggag YA, El-Ashmawy NE, Okasha KM. Is hesperidin essential for prophylaxis and treatment of COVID-19 Infection? Med Hypotheses 2020;144:109957.

10.1016/j.mehy.2020.10995732531538PMC7274964
31

Joshi RS, Jagdale SS, Bansode SB, Shankar SS, Tellis MB, Pandya VK, et al. Discovery of potential multi-target-directed ligands by targeting host-specific SARS-CoV-2 structurally conserved main protease. J Biomol Struct Dyn 2020;39:3099-114.

10.1080/07391102.2020.176013732329408PMC7212545
32

Kandeil A, Mostafa A, Kutkat O, Moatasim Y, Al‐karmalawy AA, Rashad AA, et al. Bioactive polyphenolic compounds showing strong antiviral activities against severe acute respiratory syndrome coronavirus 2. Pathogens 2021;10:758.

10.3390/pathogens1006075834203977PMC8232731
33

Panasiak W, Wleklik M, Oraczewska A, Luczak M. Influence of flavonoids on combined experimental infections with EMC virus and Staphylococcus aureus in mice. Acta Microbiol Pol 1989;38:185-8.

Information
  • Publisher :The Korean Society for Microbiology and The Korean Society of Virology
  • Publisher(Ko) :대한미생물학회‧대한바이러스학회
  • Journal Title :JOURNAL OF BACTERIOLOGY AND VIROLOGY
  • Volume : 51
  • No :4
  • Pages :200-208
  • Received Date : 2021-12-06
  • Revised Date : 2021-12-13
  • Accepted Date : 2021-12-14
  • DOI :https://doi.org/10.4167/jbv.2021.51.4.200
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