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2022 Vol.52, Issue 4 Preview Page

Original Article

Regulation in the Expression of Virulence Factors of Helicobacter pylori by the Interaction between AGS Cells and H. pylori
Yu-Ri Kim1
,
Wongwarut Boonyanugomol2
,
Won-jun An13
,
Trinh Minh Phuong13
,
Jin-Sik Park1
,
Min-Kyoung Shin1345
,
Seung-Chul Baik14
,
Woo-Kon Lee145
,
Myung-Je Cho145
,
Hyung-Lyun Kang145*
,
Myunghwan Jung1345*
1Department of Microbiology College of Medicine, Gyeongsang National University, Jinju 52727, Republic of Korea
2Department of Sciences and Liberal Arts, Mahidol University, Amnatcharoen Campus, Amnatcharoen, Thailand
3BK21 Center for Human Resource Development in the Bio-Health Industry, Department of Convergence Medical Science, Gyeongsang National University, Jinju 52727, Republic of Korea
4Institute of Health Science, Gyeongsang National University, Jinju 52727, Republic of Korea
5Research Institute of Life Science, Gyeongsang National University, Jinju 52828, Republic of Korea
*gangssi@gnu.ac.kr
*mjung@gnu.ac.kr
31 December 2022. pp. 160-169
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Abstract

Helicobacter pylori (H. pylori) was defined as a Class 1 pathogenic carcinogen by WHO causing chronic inflammation in the stomach, thereby increasing the risk of gastric cancer. Various virulence factors are involved in the mechanism of gastric cancer caused by H. pylori infection. These virulence factors usually show different expression levels depending on the environment of H. pylori, which can affect the risk of gastric cancer. In this study, the differences in the expression levels of major virulence factors of H. pylori depending on the environment were investigated by comparing expression levels of H. pylori cultured with AGS or alone. As a result, there was no difference in the expression of adhesins of alpA, sabA, and babA even after co-culture with AGS cells. In addition, the co-culture environment did not induce a difference in the expression levels of flaA and ureB. On the other hand, H. pylori co-cultured with AGS cells showed low expression levels of cagA, groEL, and oipA and high expression of vacA compared to H. pylori cultured alone. Our results suggest that not only the presence or absence of virulence factor genes but also differences in expression levels should be considered when evaluating the risk of gastric cancer after H. pylori infection based on the virulence factors.

Keywords
H. pylori
Virulence factors
Gene expression
AGS cells
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Information
  • Publisher :The Korean Society for Microbiology
  • Publisher(Ko) :대한미생물학회
  • Journal Title :JOURNAL OF BACTERIOLOGY AND VIROLOGY
  • Volume : 52
  • No :4
  • Pages :160-169
  • Received Date :2022. 11. 30
  • Revised Date :2022. 12. 07
  • Accepted Date : 2022. 12. 08
  • DOI :https://doi.org/10.4167/jbv.2022.52.4.160
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