Saucernetin-8 from Schisandra chinensis as a Potent Antiviral Agent Against Coxsackievirus B3

Hwa-Jung Choi

doi:10.4167/jbv.2024.54.1.050

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2024 Vol.54, Issue 1 Preview Page

Original Article

Saucernetin-8 from Schisandra chinensis as a Potent Antiviral Agent Against Coxsackievirus B3

31 March 2024. pp. 50-61

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Abstract

Various plants have been studied for their therapeutic potential to treat virus- induced diseases. This study aimed to investigate in vitro anti-coxsackievirus B3 (CVB3) effect of saucernetin-8 isolated from Schisandra chinensis and mode of its action. The action of saucernetin-8 on CVB3 proliferation, the effect of saucernetin-8 on VP3 protein, mitochondrial reactive oxygen species (mtROS) and expression levels of STING/p-STING/IRF3/p-IRF3 were investigated by RT-qPCR, immunofluorescence and Western blot. Saucernetin-8 exhibited over 60% inhibition of CVB3-induced cytotoxicity at a concentration of 0.4 μg/mL. Saucernetin-8 completely suppressed viral genome replication at a concentration of 10 μg/mL. Furthermore, saucernetin-8 inhibited the expression of VP3, the capsid protein of CVB3, by approximately 50% at a concentration of 10 μg/mL. Immunofluorescence assay showed that saucernetin-8 inhibited VP3 expression by approximately 50%. Saucernetin-8 increased mtROS levels in CVB3-infected Vero cells. The STING/IRF3 pathway is activated in cells treated with saucernetin-8. Therefore, saucernetin-8 is a candidate for development of antivirals against CVB3.

Keywords

Coxsackievirus B3

Saucernetin-8

Antiviral activity

Mitochondria reactive oxygen species

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Information

Publisher :The Korean Society for Microbiology and The Korean Society of Virology
Publisher(Ko) :대한미생물학회‧대한바이러스학회
Journal Title :JOURNAL OF BACTERIOLOGY AND VIROLOGY
Volume : 54
No :1
Pages :50-61
Received Date : 2024-03-23
Revised Date : 2024-04-02
Accepted Date : 2024-04-03
DOI :https://doi.org/10.4167/jbv.2024.54.1.050

JOURNAL OF BACTERIOLOGY AND VIROLOGY ISSN:1598-2467(Print) 2093-0429(Online)

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