Comparison of Microbial Detection of Hemodialysis Water in Reasoner’s 2A Agar (R2A) and Trypticase Soy Agar (TSA)

Sang-Hyun Park; Young-Hyeon Lee; Min-Ho Yeo; Hi-Ryun Lee; Hye-Sook Kim; Kyung-Soo Chang

doi:10.4167/jbv.2021.51.2.079

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2021 Vol.51, Issue 2 Preview Page

Original Article

Comparison of Microbial Detection of Hemodialysis Water in Reasoner’s 2A Agar (R2A) and Trypticase Soy Agar (TSA)

30 June 2021. pp. 79-88

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Abstract

The quality management of dialysis water used as dialysis fluid is important for patients exposed to large amounts of water. The treatment of dialysis water causes chemical and microbiological contamination. Dialysis water contaminated with bacteria causes various diseases and inflammatory reactions due to the inflow of toxins into the body. Consequently, the aim of this study was to understand the sensitivity of agar for the detection of bacteria in dialysis water, the seasonal characteristics of bacterial culture, and bacterial identification. In all, 420 samples of dialysis water collected from a hospital between September 2017 and August 2018 were cultured at clinical laboratories. The bacterial growth rate of R2A was 99 cases (23.5%), and that of TSA was 47 cases (11.1%). R2A was more sensitive than TSA for samples incubated above 1 CFU/ml in hemodialysis, and TSA was more sensitive than R2A for samples incubated above 50 CFU/ml. The morphological characteristics of the microorganisms were confirmed by gram staining 188 strains of 30 isolates from the specimens. In R2A, Gram-positive bacteria were isolated in 33.3% (n = 42), Gram-negative bacteria were isolated in 56.3% (n = 71), and fungal strains were isolated in 10.3% (n = 13). In TSA, Gram-positive bacteria were isolated in 33.8% (n = 21), Gram-negative bacteria were isolated in 64.5% (n = 40), and fungal strains were isolated in 1.6% (n = 1). In addition, seasonal distinctions were observed in microbial cultures.

Keywords

Healthcare-associated infection

HAI

Hemodialysis water

R2A

TSA

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Information

Publisher :The Korean Society for Microbiology and The Korean Society of Virology
Publisher(Ko) :대한미생물학회‧대한바이러스학회
Journal Title :JOURNAL OF BACTERIOLOGY AND VIROLOGY
Volume : 51
No :2
Pages :79-88
Received Date : 2021-05-24
Revised Date : 2021-06-28
Accepted Date : 2021-06-29
DOI :https://doi.org/10.4167/jbv.2021.51.2.079

JOURNAL OF BACTERIOLOGY AND VIROLOGY ISSN:1598-2467(Print) 2093-0429(Online)

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