Streptomyces Extract Inhibits Enterovirus 71 Replication by Activation of PKB/AKT Signaling

Bo-Young Jeong; Hong-Ki Kim; Byung-Kwan Lim

doi:10.4167/jbv.2022.52.3.094

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2022 Vol.52, Issue 3 Preview Page Next Page

Original Article

Streptomyces Extract Inhibits Enterovirus 71 Replication by Activation of PKB/AKT Signaling

30 September 2022. pp. 94-102

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Abstract

Enterovirus 71 (EV71) is a main pathogen of hand-foot, and mouth disease (HFMD) in children and adults. HFMD is a disease that causes small blisters in the mouth and hands and feet. Although blisters are usually disappeared one to two weeks after infection, HFMD is a serious disease that can lead to encephalitis and manic diseases depending on the patient. However, a representative vaccine and treatment for HFMD have not been developed yet. In this study, we investigated the antiviral effect of Streptomyces sp. zx10-19 (KH29) extract (0.1 ~ 100 μg/㎖) using EV71 infected HeLa cells. KH29 extract (100 μg/㎖) treatment significantly inhibited expression of EV71 capsid protein VP1 and cleavage of translation initiation factor eIF4G1. In addition, PKB/AKT activity was significantly increased by KH29 extract treatment. In the reverse transcription-PCR, KH29 extract treatment significantly inhibited EV71 a positive and negative-strand RNA genome amplification at 100 ug/ml. Moreover, the downstream signal molecule GSK3-beta and NF-κB phosphorylation were significantly increased following AKT activation in KH29 extract treatment. These results suggest that KH29 extract may increases cell survival through AKT signaling and effectively inhibit the proliferation of EV71, which will be used as an effective substance for the development of therapeutic agents for EV71-induced HFMD.

Keywords

Enterovirus 71

Hand-foot and mouth disease

Actinomycetes

Antiviral drug

Protein kinase B

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Information

Publisher :The Korean Society for Microbiology and The Korean Society of Virology
Publisher(Ko) :대한미생물학회‧대한바이러스학회
Journal Title :JOURNAL OF BACTERIOLOGY AND VIROLOGY
Volume : 52
No :3
Pages :94-102
Received Date : 2022-07-04
Revised Date : 2022-09-05
Accepted Date : 2022-09-07
DOI :https://doi.org/10.4167/jbv.2022.52.3.094

JOURNAL OF BACTERIOLOGY AND VIROLOGY ISSN:1598-2467(Print) 2093-0429(Online)

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