Evaluation of Commercial Immunochromatographic Test Kits for the Detection of Canine Distemper Virus

Dong-Kun Yang; Ha-Hyun Kim; Jienny Lee; Miryeon Ji; Subin Oh; Hyang-Sim Lee; Bang-Hun Hyun

doi:10.4167/jbv.2020.50.2.117

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2020 Vol.50, Issue 2 Preview Page Next Page

Original Article

Evaluation of Commercial Immunochromatographic Test Kits for the Detection of Canine Distemper Virus

30 June 2020. pp. 117-123

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Abstract

The rapid diagnosis of canine distemper virus (CDV) helps to determine the treatment of dogs in veterinary clinics. We evaluated the performance of seven commercial rapid immunochromatographic test (RICT) kits for the detection of CDV. Six core dog viral pathogens (canine adenovirus type 1 and 2, canine coronavirus, canine parainfluenza virus, canine parvovirus, and rabies virus), five CDV strains (CD1901, Lederle, Rockborn, Onderstepoort, and Synder Hill), and three bacteria (Bordetella bronchiseptica, Leptospira canicola, and Staphylococus aureus) were used to determine the cross-reactivity and detection limits of the kits. The seven commercial RICT kits did not yield positive results with the six dog viruses or the three bacteria. All the RICT kits for CDV detected the Korean CDV isolate. The detection limits of the RICT kits for the Korean CDV isolate, CD1901, belonging to Asia 1 genotype ranged from 10^3.0 to 10^4.0 TCID₅₀/mL. There was an average difference of 1.1 in scores judged by eye between four CDV vaccine strains and CD1901 strain. Therefore, the RICT kits enable the detection of CDV vaccine strains, but need to be improved to detect CDV circulating in dog populations in Korea.

Keywords

Canine distemper virus

Chromatography

Diagnosis

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Information

Publisher :The Korean Society for Microbiology and The Korean Society of Virology
Publisher(Ko) :대한미생물학회‧대한바이러스학회
Journal Title :JOURNAL OF BACTERIOLOGY AND VIROLOGY
Volume : 50
No :2
Pages :117-123
Received Date : 2020-01-01
Revised Date : 2020-05-31
Accepted Date : 2020-06-01
DOI :https://doi.org/10.4167/jbv.2020.50.2.117

JOURNAL OF BACTERIOLOGY AND VIROLOGY ISSN:1598-2467(Print) 2093-0429(Online)

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