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2020 Vol.50, Issue 3 Preview Page

Original Article

In Vitro Virucidal Effect of Povidone-Iodine Against SARS-CoV-2
Kyeong Ryeol Shin1†
,
Kyunghee Kwak2†
,
Chunguang Cui1
,
Joon-Yong Bae1
,
Woosung Hong2†
,
Man-Seong Park1†
1Department of Microbiology, Institute for Viral Diseases, College of Medicine, Korea University
2Mundipharma Korea Ltd. Medical Department
*WooSung.Hong@mundipharma.co.kr; manseong.park@gmail.com
† These authors contributed equally to this work.
30 September 2020. pp. 195-202
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Abstract

As of September 2020, SARS-CoV-2 has infected over 30 million people worldwide, and the death toll has now risen to 950,000. Given that Povidone-iodine (PVP-I) had consistently been showing the virucidal efficacy against various types of viruses, such as SARS-CoV, MERS-CoV, and Ebola, we conducted this study to figure out the virucidal effect against SARS-CoV-2 by using a viral plaque assay. We performed Kill-Time assays to assess the viral inactivation of SARS-CoV-2 contaminants after the application of the PVP-I product (Betadine® Throat Spray, PVP-I 0.45%). This test consisted of clean and dirty conditions and was designed to check the viral titers at a contact time of 60 seconds, which were evaluated by plaque-reduction rates in Vero cells. This PVP-I product fully achieved ≥4 log10 reductions in viral titers under both clean and dirty conditions. This level of reduction, ≥4 log10 (99.99%), in viral titers presented to be effective in terms of virucidal efficacy, according to the European standards, EN14476. This study revealed the virucidal efficacy of Betadine® Throat Spray against SARS-CoV-2 virus. Given that the convenience and availability of this product, we think that it may contribute to inhibit viral infection and transmissibility as an active type of personal protective equipment (PPE) by managing the hygiene of patients and medical professionals.

Keywords
COVID-19
SARS-CoV-2
Plaque
Povidone-iodine
Virucidal effect
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Information
  • Publisher :The Korean Society for Microbiology and The Korean Society of Virology
  • Publisher(Ko) :대한미생물학회‧대한바이러스학회
  • Journal Title :JOURNAL OF BACTERIOLOGY AND VIROLOGY
  • Volume : 50
  • No :3
  • Pages :195-202
  • Received Date : 2020-08-18
  • Revised Date : 2020-09-20
  • Accepted Date : 2020-09-22
  • DOI :https://doi.org/10.4167/jbv.2020.50.3.195
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