Effects of Exogenous N-Acyl-Homoserine Lactones on Biofilm Formation and Motility in Acinetobacter nosocomialis

Surya Surendran; Bindu Subhadra; Kyungho Woo; Ho Sung Park; Dong Ho Kim; Man Hwan Oh; Chul Hee Choi

doi:10.4167/jbv.2020.50.2.097

All Issue

2020 Vol.50, Issue 2 Preview Page Next Page

Original Article

Effects of Exogenous N-Acyl-Homoserine Lactones on Biofilm Formation and Motility in Acinetobacter nosocomialis

30 June 2020. pp. 97-106

PDF XML

Abstract

One of the major factors contributing to drug resistance in Acinetobacter nosocomialis infections is biofilm development, which is facilitate by quorum-sensing (QS) systems. Quorum sensing by the LuxI and LuxR homologues, AnoI and AnoR, in A. nosocomialis plays a role in biofilm formation and motility of this pathogenic bacterium. The aim of this study was to evaluate the effects of exogenous N-acyl-homoserine lactones (AHLs) on the regulation of biofilm and motility of A. nosocomialis and anoI-deletion mutant. We found that anoR mRNA expression levels in the anoI-deletion mutant were increased in the presence of different types of AHLs compared with that in the absence of exogenous AHL. Among AHLs, C12-HSL appeared to exert the greatest stimulatory effect on biofilm formation and motility. Notably, the anoI-deletion mutant also exhibited significant decreases in expression of the biofilm- and motility-related genes, csuC, csuD and pilT, decreases that were attenuated by addition of exogenous AHLs. Combining the AHL C12-HSL with C6-HSL or C10-HSL exerted synergistic effects that restored the motility phenotype in the anoI-deletion mutant. Taken together, our data demonstrate that C12-HSL may act as an important signaling molecule in A. nosocomialis through regulation of biofilm formation and cell motility, potentially providing a new target for the control of A. nosocomialis infections.

Keywords

Quorum sensing

Exogenous AHLs

Biofilm formation

Motility

A. nosocomialis

References

Qi C, Malczynski M, Parker M, Scheetz MH. Characterization of genetic diversity of carbapenem-resistant Acinetobacter baumannii clinical strains collected from 2004 to 2007. J Clin Microbiol 2008;46:1106-9.

10.1128/JCM.01877-0718216212PMC2268351

Wang X, Chen T, Yu R, Lü X, Zong Z. Acinetobacter pittii and Acinetobacter nosocomialis among clinical isolates of the Acinetobacter calcoaceticus-baumannii complex in Sichuan, China. Diagn Microbiol Infect Dis 2013;76:392-5.

10.1016/j.diagmicrobio.2013.03.02023639796

Peleg AY, Seifert H, Paterson DL. Acinetobacter baumannii: emergence of a successful pathogen. Clin Microbiol Rev 2008;21:538-82.

10.1128/CMR.00058-0718625687PMC2493088

Withers H, Swift S, Williams P. Quorum sensing as an integral component of gene regulatory networks in Gram-negative bacteria. Curr Opin Microbiol 2001;4:186-93.

10.1016/S1369-5274(00)00187-9

Bassler BL, Wright M, Showalter RE, Silverman MR. Intercellular signalling in Vibrio harveyi: sequence and function of genes regulating expression of luminescence. Mol Microbiol 1993;9:773-86.

10.1111/j.1365-2958.1993.tb01737.x8231809

Miller MB, Bassler BL. Quorum sensing in bacteria. Annu Rev Microbiol 2001;55:165-99.

10.1146/annurev.micro.55.1.16511544353

Oh MH, Choi CH. Role of LuxIR Homologue AnoIR in Acinetobacter nosocomialis and the Effect of Virstatin on the Expression of anoR Gene. J Microbiol Biotechnol 2015;25:1390-400.

10.4014/jmb.1504.0406925975610

Zhu YL, Hou HM, Zhang GL, Wang YF, Hao HS. AHLs Regulate Biofilm Formation and Swimming Motility of Hafnia alvei H4. Front Microbiol 2019;10:1330.

10.3389/fmicb.2019.0133031275267PMC6593095

Lee HW, Koh YM, Kim J, Lee JC, Lee YC, Seol SY, et al. Capacity of multidrug-resistant clinical isolates of Acinetobacter baumannii to form biofilm and adhere to epithelial cell surfaces. Clin Microbiol Infect 2008;14:49-54.

10.1111/j.1469-0691.2007.01842.x18005176

Armbruster CR, Parsek MR. New insight into the early stages of biofilm formation. Proc Natl Acad Sci USA 2018;115:4317-9.

10.1073/pnas.180408411529632199PMC5924939

Tolker-Nielsen T. Biofilm Development. Microbiol Spectr 2015;3:MB-0001-2014.

10.1128/microbiolspec.MB-0001-201426104692

Dunne WM Jr. Bacterial adhesion: seen any good biofilms lately? Clin Microbiol Rev 2002;15:155-66.

10.1128/CMR.15.2.155-166.200211932228PMC118072

Fux CA, Costerton JW, Stewart PS, Stoodley P. Survival strategies of infectious biofilms. Trends Microbiol 2005;13:34-40.

10.1016/j.tim.2004.11.01015639630

Nemec A, Krizova L, Maixnerova M, van der Reijden TJ, Deschaght P, Passet V, et al. Genotypic and phenotypic characterization of the Acinetobacter calcoaceticus-Acinetobacter baumannii complex with the proposal of Acinetobacter pittii sp. nov. (formerly Acinetobacter genomic species 3) and Acinetobacter nosocomialis sp. nov. (formerly Acinetobacter genomic species 13TU). Res Microbiol 2011;162:393-404.

10.1016/j.resmic.2011.02.00621320596

Oh MH, Lee JC, Kim J, Choi CH, Han K. Simple Method for Markerless Gene Deletion in Multidrug-Resistant Acinetobacter baumannii. Appl Environ Microbiol 2015;81:3357-68.

10.1128/AEM.03975-1425746991PMC4407223

Eberl L. N-acyl homoserinelactone-mediated gene regulation in gram-negative bacteria. Syst Appl Microbiol 1999;22:493-506.

10.1016/S0723-2020(99)80001-0

Saipriya K, Swathi CH, Ratnakar KS, Sritharan V. Quorum-sensing system in Acinetobacter baumannii: a potential target for new drug development. J Appl Microbiol 2020;128:15-27.

10.1111/jam.1433031102552

Rasmussen TB, Givskov M. Quorum sensing inhibitors: a bargain of effects. Microbiology 2006;152:895-904.

10.1099/mic.0.28601-016549654

Li Z, Nair SK. Quorum sensing: how bacteria can coordinate activity and synchronize their response to external signals? Protein Sci 2012;21:1403-17.

10.1002/pro.213222825856PMC3526984

Rémy B, Mion S, Plener L, Elias M, Chabrière E, Daudé D. Interference in Bacterial Quorum Sensing: A Biopharmaceutical Perspective. Front Pharmacol 2018;9:203.

10.3389/fphar.2018.0020329563876PMC5845960

Gurich N, González JE. Role of quorum sensing in Sinorhizobium meliloti-Alfalfa symbiosis. J Bacteriol 2009;191:4372-82.

10.1128/JB.00376-0919395488PMC2698488

Andersson RA, Eriksson AR, Heikinheimo R, Mäe A, Pirhonen M, Kõiv V, et al. Quorum sensing in the plant pathogen Erwinia carotovora subsp. carotovora: the role of expR (Ecc). Mol Plant Microbe Interact 2000;13:384-93.

10.1094/MPMI.2000.13.4.38410755301

Nasser W, Bouillant ML, Salmond G, Reverchon S. Characterization of the Erwinia chrysanthemi expI-expR locus directing the synthesis of two N-acyl-homoserine lactone signal molecules. Mol Microbiol 1998;29:1391-405.

10.1046/j.1365-2958.1998.01022.x9781877

Information

Publisher :The Korean Society for Microbiology and The Korean Society of Virology
Publisher(Ko) :대한미생물학회‧대한바이러스학회
Journal Title :JOURNAL OF BACTERIOLOGY AND VIROLOGY
Volume : 50
No :2
Pages :97-106
Received Date : 2020-04-24
Revised Date : 2020-05-20
Accepted Date : 2020-05-25
DOI :https://doi.org/10.4167/jbv.2020.50.2.097

JOURNAL OF BACTERIOLOGY AND VIROLOGY ISSN:1598-2467(Print) 2093-0429(Online)

All Issue