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2022 Vol.52, Issue 1 Preview Page

Original Article

Establishment of Multiplex RT-PCR for Differentiating between the CD1901 and Lederle Strains of Canine Distemper Virus
Dong-Kun Yang1†
,
Yu-Ri Park1
,
Yesel Park1
,
Bang-Hun Hyun1
1Viral Disease Division, Animal and Plant Quarantine Agency, MAFRA, Gimcheon 39660, Republic of Korea
*yangdk@korea.kr
31 March 2022. pp. 11-19
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Abstract

Canine distemper virus (CDV) is a life-threatening pathogen in dogs. Clinical, pathological and molecular methods are required to diagnose CDV infection, and it is important to differentiate between the Korean CDV strain CD1901 and Lederle CDV vaccine strain. Therefore, in this study, we used multiplex reverse- transcription polymerase chain reaction (RT-PCR) to differentiate between the CD1901 and Lederle strains. A primer set was designed based on the CDV nucleoprotein gene and nucleotide sequence variation in the fusion (F) gene. First, 224-bp DNA bands were amplified from viral RNA of the CD1901 and Lederle strains. Then, 428- and 326-bp DNA bands were amplified in the CD1901 and Lederle strain, respectively. The multiplex RT-PCR detection limits were 2.53 and 0.8 median tissue culture infectious dose/reaction for the CD1901 and Lederle strains, respectively. No cross-reactions were detected in non-CDV reference viruses, including rabies virus, parvovirus, canine adenovirus types 1 and 2, and parainfluenza virus. The results indicate that our one-step multiplex RT-PCR is useful for differentiating between wildtype and vaccine CDV strains.

Keywords
Canine distemper virus
multiplex RT-PCR
differentiation
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Information
  • Publisher :The Korean Society for Microbiology and The Korean Society of Virology
  • Publisher(Ko) :대한미생물학회‧대한바이러스학회
  • Journal Title :JOURNAL OF BACTERIOLOGY AND VIROLOGY
  • Volume : 52
  • No :1
  • Pages :11-19
  • Received Date : 2022-01-07
  • Revised Date : 2022-03-03
  • Accepted Date : 2022-03-22
  • DOI :https://doi.org/10.4167/jbv.2022.52.1.011
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