Rapid and Simultaneous Detection of Dengue and Chikungunya Viruses by a Multiplex Lateral Flow Assay Using Ficolin-1,  One of Human Innate Immune Defense Proteins

So Yeon Yi; Jungsun Kwon; Jae Hoon Lee; Kyungah Yoon; Yong Beom Shin; Kyoungsook Park

doi:10.4167/jbv.2022.52.1.001

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2022 Vol.52, Issue 1 Next Page

Original Article

Rapid and Simultaneous Detection of Dengue and Chikungunya Viruses by a Multiplex Lateral Flow Assay Using Ficolin-1, One of Human Innate Immune Defense Proteins

31 March 2022. pp. 1-10

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Abstract

The infection of dengue virus (DENV) and chikungunya virus (CHIKV) can cause global public health problems, many of which are undifferentiated. Thus, their differential diagnosis is critical to proper patient management. In this study, we present a novel multiplex diagnostic system based on the lateral flow assay (LFA) using ficolin-1 and two-color latex beads labeled with antibodies to detect the dengue virus (DENV) and chikungunya virus (CHIKV) in a single strip. We investigated the binding of ficolin-1, a human innate immune system defense protein, to the viral envelope protein (EP) and developed the LFA system that contains the capturing agent, ficolin-1, which was immobilized on the test line. Our diagnostic system could differentially detect DENV-2 and CHIKV based on the color of the detecting agent in the test line of the strip. The limit of detection (LOD) by naked-eye observation in the multiplex LFA for viral EPs was 25 nM and that for the whole virus was 1 ´ 10⁶ TCID₅₀/mL per strip. Thus, multiplex LFA using ficolin-1 provides a rapid method for simultaneously detecting DENV and CHIKV and can be employed to monitor the status of circulating mosquitoes in a region at risk for DENV and/or CHIKV outbreak.

Keywords

Chikungunya virus

Dengue virus

Ficolin-1

Innate immune system

Multiplex lateral flow assay

References

Glushakova LG, Alto BW, Kim MS, Wiggins K, Eastmond B, Moussatche P, et al. Optimization of cationic (Q)-paper for detection of arboviruses in infected mosquitoes. J Virol Methods 2018;261:71-9.

10.1016/j.jviromet.2018.08.00430099053PMC6168196

Cecilia D, Kakade M, Alagarasu K, Patil J, Salunke A, Parashar D, et al. Development of a multiplex real-time RT-PCR assay for simultaneous detection of dengue and chikungunya viruses. Arch Virol 2015;160: 323-7.

10.1007/s00705-014-2217-x25233940

Mukherjee S, Dutta SK, Sengupta S, Tripathi A. Evidence of dengue and chikungunya virus co-infection and circulation of multiple dengue serotypes in a recent Indian outbreak. Eur J Clin Microbiol Infect Dis 2017;36: 2273-9.

10.1007/s10096-017-3061-128756561

Dash PK, Parida M, Santhosh SR, Saxena P, Srivastava A, Neeraja M, et al. Development and evaluation of a 1-step duplex reverse transcription polymerase chain reaction for differential diagnosis of chikungunya and dengue infection. Diagn Microbiol Infect Dis 2008;62:52-7.

10.1016/j.diagmicrobio.2008.05.00218583086

Lopez-Jimena B, Wehner S, Harold G, Bakheit M, Frischmann S, Bekaert M, et al. Development of a single-tube one-step RT-LAMP assay to detect the Chikungunya virus genome. PLoS Negl Trop Dis 2018;12:e0006448.

10.1371/journal.pntd.000644829813065PMC5973553

Rianthavorn P, Wuttirattanakowit N, Prianantathavorn K, Limpaphayom N, Theamboonlers A, Poovorawan Y. Evaluation of a rapid assay for detection of IgM antibodies to chikungunya. Southeast Asian J Trop Med Public Health 2010;41:92-6.

Kam YW, Lee WW, Simarmata D, Harjanto S, Teng TS, Tolou H, et al. Longitudinal analysis of the human antibody response to Chikungunya virus infection: implications for serodiagnosis and vaccine development. J Virol 2012;86:13005-15.

10.1128/JVI.01780-1223015702PMC3497641

Bhatnagar S, Kumar P, Mohan T, Verma P, Parida MM, Hoti SL, et al. Evaluation of multiple antigenic peptides based on the Chikungunya E2 protein for improved serological diagnosis of infection. Viral Immunol 2015;28:107-12.

10.1089/vim.2014.003125412351

Tripathi NK, Priya R, Shrivastava A. Production of recombinant Chikungunya virus envelope 2 protein in Escherichia coli. Appl Microbiol Biotechnol 2014;98:2461-71.

10.1007/s00253-013-5426-424337252

Holmskov U, Thiel S, Jensenius JC. Collections and ficolins: humoral lectins of the innate immune defense. Annu Rev Immunol 2003;21:547-78.

10.1146/annurev.immunol.21.120601.14095412524383

Favier AL, Gout E, Reynard O, Ferraris O, Kleman JP, Volchkov V, et al. Enhancement of Ebola Virus Infection via Ficolin-1 Interaction with the Mucin Domain of GP Glycoprotein. J Virol 2016;90:5256-69.

10.1128/JVI.00232-1626984723PMC4934759

Gout E, Garlatti V, Smith DF, Lacroix M, Dumestre-Pérard C, Lunardi T, et al. Carbohydrate recognition properties of human ficolins: glycan array screening reveals the sialic acid binding specificity of M-ficolin. J Biol Chem 2010;285:6612-22.

10.1074/jbc.M109.06585420032467PMC2825457

Liu Y, Endo Y, Iwaki D, Nakata M, Matsushita M, Wada I, et al. Human M-ficolin is a secretory protein that activates the lectin complement pathway. J Immunol 2005;175:3150-6.

10.4049/jimmunol.175.5.315016116205

Kjaer TR, Hansen AG, Sørensen UB, Holm AT, Sørensen GL, Jensenius JC, et al. M-ficolin binds selectively to the capsular polysaccharides of Streptococcus pneumoniae serotypes 19B and 19C and of a Streptococcus mitis strain. Infect Immun 2013;81:452-9.

10.1128/IAI.01148-1223184524PMC3553806

Liu J, Ali MA, Shi Y, Zhao Y, Luo F, Yu J, et al. Specifically binding of L-ficolin to N-glycans of HCV envelope glycoproteins E1 and E2 leads to complement activation. Cell Mol Immunol 2009;6:235-44.

10.1038/cmi.2009.3219728924PMC4002714

Metz SW, Geertsema C, Martina BE, Andrade P, Heldens JG, van Oers MM, et al. Functional processing and secretion of Chikungunya virus E1 and E2 glycoproteins in insect cells. Virol J 2011;8:353.

10.1186/1743-422X-8-35321762510PMC3162542

Reed LJ, Muench H. A simple method of estimating fifty per cent endpoints. Am J Hygiene 1938;27:493-7.

10.1093/oxfordjournals.aje.a118408

Thiemmeca S, Tamdet C, Punyadee N, Prommool T, Songjaeng A, Noisakran S, et al. Secreted NS1 Protects Dengue Virus from Mannose-Binding Lectin-Mediated Neutralization. J Immunol 2016;197:4053-65.

10.4049/jimmunol.160032327798151PMC5123808

Oh YK, Joung HA, Han HS, Suk HJ, Kim MG. A three-line lateral flow assay strip for the measurement of C-reactive protein covering a broad physiological concentration range in human sera. Biosens Bioelectron 2014;61:285-9.

10.1016/j.bios.2014.04.03224906087

Watanabe Y, Ito T, Ibrahim MS, Arai Y, Hotta K, Phuong HV, et al. A novel immunochromatographic system for easy-to-use detection of group 1 avian influenza viruses with acquired human-type receptor binding specificity. Biosens Bioelectron 2015;65:211-9.

10.1016/j.bios.2014.10.03625461160PMC7125538

Lee S, Mehta S, Erickson D. Two-Color Lateral Flow Assay for Multiplex Detection of Causative Agents Behind Acute Febrile Illnesses. Anal Chem 2016;88:8359-63.

10.1021/acs.analchem.6b0182827490379PMC5396465

Information

Publisher :The Korean Society for Microbiology and The Korean Society of Virology
Publisher(Ko) :대한미생물학회‧대한바이러스학회
Journal Title :JOURNAL OF BACTERIOLOGY AND VIROLOGY
Volume : 52
No :1
Pages :1-10
Received Date : 2021-10-06
Revised Date : 2022-01-02
Accepted Date : 2022-01-07
DOI :https://doi.org/10.4167/jbv.2022.52.1.001

JOURNAL OF BACTERIOLOGY AND VIROLOGY ISSN:1598-2467(Print) 2093-0429(Online)

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