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2023 Vol.53, Issue 2 Preview Page

Original Article

Spa Typing and Virulence Attributes of Multidrug-Resistant Staphylococcus aureus in Goat: A Veterinary Hospital-Based Cross-Sectional Study
Avijit Dutta1†
,
Chandan Nath1*†
,
Md. Moktadir Billah Reza2
,
Tridip Das3
,
Eaftekhar Ahmed Rana1
,
Ayesha Sultana4
,
Mohammad Shah Jalal5
1Department of Microbiology and Veterinary Public Health, Chattogram Veterinary and Animal Sciences University, Chattogram 4225, Bangladesh
2Department of Medicine and Surgery, Chattogram Veterinary and Animal Sciences University, Chattogram 4225, Bangladesh
3School of Agricultural, Environmental and Veterinary Sciences, Faculty of Sciences and Health, Charles Sturt University, Wagga Wagga, NSW-2650, Australia
4Department of Pathology and Parasitology, Chattogram Veterinary and Animal Sciences University, Chattogram 4225, Bangladesh
5Department of Pathology and Microbiology, Faculty of Veterinary Medicine, University of Montreal, Québec H3C 5J9, Canada
*chandannath227@gmail.com
†These authors equally contributed to this work.
31 December 2023. pp. 150-160
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Abstract

Staphylococcus aureus (S. aureus) not only causes significant loss of livestock production but also has potential public health risks. This study was conducted with the objective of characterizing the antibiogram of S. aureus from goats attending a Teaching Veterinary Hospital in Bangladesh. We aimed to study the spa type, potential virulence factor(s), and the presence of methicillin and vancomycin resistance genes in the isolates. From a total of 200 goat nasal swab samples S. aureus was confirmed by PCR. Antimicrobial susceptibility testing was performed using the disc diffusion method followed by mecA and vanA gene PCR. Staphylococcal protein A (spa) typing was conducted for selected isolates. A set of major virulence determinants, including sea, seb, pvl, and tst were investigated by PCR. A total 11.5% (n=23) of S. aureus isolates were obtained. Ciprofloxacin resistance was the highest (100%; n=23) followed by cefoxitin (74%; n=17). About 91% of the isolates (n=21) were multidrug-resistant (resistant to ≥3 antimicrobial classes). One (6%) isolate was MRSA harboring mecA. Among 13 vancomycin-resistant isolates, three (23%) were vanA-positive. Of the virulence genes screened, sea was recorded in four (17%) isolates. The spa typing of two randomly selected strains revealed that one isolate belonged to spa type t5259, and the other seemed to be novel. Goats having cutaneous and mucosal lesions had higher S. aureus carriage (P=0.01) with a significant variation across the breeds (P= 0.02). This study provides insights into S. aureus strains circulating in the goat population in Bangladesh.

Keywords
S. aureus
MDR
PCR
Spa typing
Goat
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Information
  • Publisher :The Korean Society for Microbiology and The Korean Society of Virology
  • Publisher(Ko) :대한미생물학회‧대한바이러스학회
  • Journal Title :JOURNAL OF BACTERIOLOGY AND VIROLOGY
  • Volume : 53
  • No :2
  • Pages :150-160
  • Received Date : 2023-09-09
  • Revised Date : 2023-11-14
  • Accepted Date : 2023-11-15
  • DOI :https://doi.org/10.4167/jbv.2023.53.2.150
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