Recombinant ACE2-Ig Fusion Protein Neutralizes SARS-CoV-2

Jin-Woo Song; Young-Sang Koh

doi:10.4167/jbv.2022.52.1.039

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2022 Vol.52, Issue 1 Preview Page

Letter to the Editor

Recombinant ACE2-Ig Fusion Protein Neutralizes SARS-CoV-2

31 March 2022. pp. 39-40

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Abstract

A novel coronavirus (SARS-CoV-2) is the causative agent for the COVID-19 pandemic, and needs for effective antiviral drugs have been demanded. Angiotensin-converting enzyme 2 (ACE2) has been shown to be a cellular receptor for SARS-CoV and SARS-CoV-2. For the therapeutic validation of ACE2, a recombinant protein consisting of the ACE2 extracellular domain fused to the IgG1 Fc domain (ACE2-Ig) was generated. ACE2-Ig exhibits proper pharmacological properties and binds with a high affinity to SARS-CoV and SARS-CoV-2 receptor-binding domain. Further, it neutralizes virus with SARS-CoV and SARS-CoV-2 spike proteins. Taken overall, these data suggest that ACE2-Ig has substantial anti-SARS-CoV-2 properties and warrant further study concerning its potential applications for the treatment of SARS-CoV-2.

Keywords

SARS-CoV-2

ACE2-Ig

Neutralization

Affinity

Receptor-binding domain

References

Lei C, Qian K, Li T, Zhang S, Fu W, Ding M, et al. Neutralization of SARS-CoV-2 spike pseudotyped virus by recombinant ACE2-Ig. Nat Commun 2020;11:2070.

10.1038/s41467-020-16048-432332765PMC7265355

Huang C, Wang Y, Li X, Ren L, Zhao J, Hu Y, et al. Clinical features of patients infected with 2019 novel coronavirus in Wuhan, China. Lancet 2020;395:497-506.

10.1016/S0140-6736(20)30183-5

Wrapp D, Wang N, Corbett KS, Goldsmith JA, Hsieh C, Abiona O, et al. Cryo-EM structure of the 2019-nCoV spike in the prefusion conformation. Science 2020;367:1260-3.

10.1126/science.abb250732075877PMC7164637

Yan R, Zhang Y, Li Y, Xia L, Guo Y, Zhou Q. Structural basis for the recognition of SARS-CoV-2 by full-length human ACE2. Science 2020;367:1444-8.

10.1126/science.abb276232132184PMC7164635

Jackson CB, Farzan M, Chen B, Choe H. Mechanisms of SARS-CoV-2 entry into cells. Nat Rev Mol Cell Biol 2022;23:3-20.

10.1038/s41580-021-00418-x34611326PMC8491763

Rice GI, Jones AL, Grant PJ, Carter AM, Turner AJ, Hooper NM. Circulating activities of angiotensin-converting enzyme, its homolog, angiotensin-converting enzyme 2, and neprilysin in a family study. Hypertension 2006;48:914-20.

10.1161/01.HYP.0000244543.91937.7917000927

Liu P, Wysocki J, Souma T, Ye M, Ramirez V, Zhou B, et al. Novel ACE2-Fc chimeric fusion provides long-lasting hypertension control and organ protection in mouse models of systemic renin angiotensin system activation. Kidney Int 2018;94:114-25.

10.1016/j.kint.2018.01.02929691064

Junker F, Gordon J, Qureshi O. Fc gamma receptors and their role in antigen uptake, presentation, and T cell activation. Front Immunol 2020;11:1393.

10.3389/fimmu.2020.0139332719679PMC7350606

Information

Publisher :The Korean Society for Microbiology and The Korean Society of Virology
Publisher(Ko) :대한미생물학회‧대한바이러스학회
Journal Title :JOURNAL OF BACTERIOLOGY AND VIROLOGY
Volume : 52
No :1
Pages :39-40
Received Date : 2022-03-07
Revised Date : 2022-03-07
Accepted Date : 2022-03-22
DOI :https://doi.org/10.4167/jbv.2022.52.1.039

JOURNAL OF BACTERIOLOGY AND VIROLOGY ISSN:1598-2467(Print) 2093-0429(Online)

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