Genetic Change of Varicella-Zoster Virus Propagated in Cell Culture in Non-Natural Conditions

Sang Hoon Yeon; Ji Seon Park; Se Hwan Kang; Chan Hee Lee

doi:10.4167/jbv.2021.51.4.178

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2021 Vol.51, Issue 4 Preview Page Next Page

Original Article

Genetic Change of Varicella-Zoster Virus Propagated in Cell Culture in Non-Natural Conditions

31 December 2021. pp. 178-188

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Abstract

Primary infection of varicella-zoster virus (VZV) causes varicella and often leads to zoster after reactivation from latency. Both varicella and zoster can be prevented by live attenuated vaccines, but the molecular mechanism of attenuation is not clearly understood. In this study, it was attempted to understand mechanism of attenuating mutation in VZV by in vitro propagation in non-natural conditions such as low temperature or non-human cell. Clinical strain YC02 was subcultured in vitro up to 60 times. Comparison of the genome sequences of YC02 variants cultured under various conditions identified specific mutations occurred in non-natural conditions. The mutations specific for low temperature culture and non-human cell culture were identified in 8 and 2 positions, respectively. Two vaccine-specific mutations in position 97748 and 106262 were identified during subculture in non-natural conditions. Genetic diversity as measured by genetic polymorphism and Shannon entropy decreased when cultured in guinea pig lung cell culture. The infectivity of YC02 cultured at low temperature appeared similar to that cultured in natural condition. On the other hands, infectivity decreased significantly when YC02 was subcultured in non-human cell. Further studies on mutations and genetic diversity of clinical strain cultured in non-natural conditions will help to elucidate the molecular mechanism of VZV attenuation.

Keywords

Varicella-zoster virus

Vaccine

Low temperature culture

Non-human cell culture

Mutation

Genetic diversity

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Information

Publisher :The Korean Society for Microbiology and The Korean Society of Virology
Publisher(Ko) :대한미생물학회‧대한바이러스학회
Journal Title :JOURNAL OF BACTERIOLOGY AND VIROLOGY
Volume : 51
No :4
Pages :178-188
Received Date : 2021-08-30
Revised Date : 2021-12-06
Accepted Date : 2021-12-06
DOI :https://doi.org/10.4167/jbv.2021.51.4.178

JOURNAL OF BACTERIOLOGY AND VIROLOGY ISSN:1598-2467(Print) 2093-0429(Online)

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