Endogenously Synthesized n-3 fatty Acids in fat-1 Transgenic Mice Suppresses B Cell Activation

Da-Hee Kim; So-Hyun Park; Sang-Myeong Lee

doi:10.4167/jbv.2022.52.4.184

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2022 Vol.52, Issue 4 Preview Page

Original Article

Endogenously Synthesized n-3 fatty Acids in fat-1 Transgenic Mice Suppresses B Cell Activation

31 December 2022. pp. 184-193

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Abstract

n-3 Polyunsaturated fatty acids (PUFAs) have been suggested as a preventive or therapeutic strategy for autoimmune or inflammatory diseases via the modulation of the activation, differentiation, and effector functions of various immune cells, especially T cells and macrophages. Despite the importance of B cells in the pathogenesis of autoimmune diseases, few studies have demonstrated their effect on B cells. In this study, we investigated the possible effects of n-3 PUFAs on B cell activation, differentiation, and isotype switching in fat-1 transgenic mice, which can convert n-6 PUFAs to n-3 PUFAs via n-3 desaturase derived from Caenorhabditis elegans. Fat-1 mice were fed a corn oil-rich diet for at least 4 weeks to increase the probability of converting n-6 PUFAs to n-3 PUFAs. Splenic B cells were then isolated, followed by stimulation with Toll-like receptor 4 or B cell receptor (BCR). Our results showed that increased n-3 PUFA levels in B cells by fat-1 overexpression inhibited B cell proliferation, cytokine secretion, such as interleukin (IL)-6, IL-10, tumor necrosis factor-α, plasma cell differentiation, and Ig isotype switching/secretion. These effects are possibly due to the suppression of Syk, Src, and AKT activation in B cells from fat-1 mice, as well as NF-κB. Collectively, our results suggest that fat-1 expression attenuates B cell responses, and n-3 PUFA supplementation can be a possible strategy to treat pathological conditions in which B cells play a key role.

Keywords

N-3 PUFAs

Fat-1

B cell

Plasma cells

Immunoglobulin

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Information

Publisher :The Korean Society for Microbiology
Publisher(Ko) :대한미생물학회
Journal Title :JOURNAL OF BACTERIOLOGY AND VIROLOGY
Volume : 52
No :4
Pages :184-193
Received Date : 2022-12-09
Revised Date : 2022-12-19
Accepted Date : 2022-12-20
DOI :https://doi.org/10.4167/jbv.2022.52.4.184

JOURNAL OF BACTERIOLOGY AND VIROLOGY ISSN:1598-2467(Print) 2093-0429(Online)

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