Comparison of Primers for Oral Mycobiome Study in Intubated Patients

Yoon Hee Choi; Soo Hyun Kim; Myoung Soo Kim; Hee Sam Na

doi:10.4167/jbv.2021.51.3.112

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2021 Vol.51, Issue 3 Preview Page Next Page

Original Article

Comparison of Primers for Oral Mycobiome Study in Intubated Patients

30 September 2021. pp. 112-119

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Abstract

In intubated patients, oral fungus including Candida rapidly can increase. Fungal infections cause a significant proportion of health care associated infections. For efficient prevention and management, characterization of the oral mycobiome is required. In this study, we tested two primers to characterize the oral mycobiome in intubated patients under mechanical ventilation support. Buccal samples from intubated patients were collected using Levine technique. Two primers (ITS3 and ITS7) targeting internal transcribed spacer (ITS)2 region was tested. Next generation sequencing was applied for mycobiome analysis. Alpha diversity, beta diversity and relative abundance was determined to compare the primer performance. The richness and evenness were significantly higher using ITS7 primer. When phylogenetic distance was included, there was no difference in richness. In beta diversity analysis, most of the samples were closely clustered suggesting that similar mycobiome structure was assigned by the tested primers. When relative abundance of the assigned taxa was compared, Candida was the most abundant genus. At the species level, similar species was assigned by both primers. However, C. sake, C. intermedia, C. parapsiolisis and Yarrowia porcina was only assigned by ITS3 primer, while Alternaria betae-kenyesis was only assigned by ITS7 primer. In intubated patients, diverse oral mycobiome was detected. Although the tested primers predicted similar mycobiome structure, there were several species that was uniquely assigned by each primer. Selecting optimum primer to characterize the oral mycobiome should provide better understanding the mycobiome community in oral cavity.

Keywords

Oral mycobiome

Intubation

ITS2

Candida

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Information

Publisher :The Korean Society for Microbiology and The Korean Society of Virology
Publisher(Ko) :대한미생물학회‧대한바이러스학회
Journal Title :JOURNAL OF BACTERIOLOGY AND VIROLOGY
Volume : 51
No :3
Pages :112-119
Received Date : 2021-07-01
Revised Date : 2021-08-05
Accepted Date : 2021-08-06
DOI :https://doi.org/10.4167/jbv.2021.51.3.112

JOURNAL OF BACTERIOLOGY AND VIROLOGY ISSN:1598-2467(Print) 2093-0429(Online)

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