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2025 Vol.55, Issue 1 Preview Page

Original Article

Mast Cells Promote Macrophage Polarization to M1-like Phenotype through a Tryptase-dependent Process
Kyung-Ah Cho1
,
Jiyun Kwon1
,
Hyeon Ju Kim1
,
So-Youn Woo1*
1Department of Microbiology, College of Medicine, Ewha Womans University, Seoul 07804, Republic of Korea
*soyounwoo@ewha.ac.kr
31 March 2025. pp. 79-90
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Abstract

Mast cells (MCs) activate and support macrophage proliferation in inflammatory microenvironments. However, the role of MCs in regulating macrophage polarization under normal physiological conditions remains poorly understood. In this study, we used conditioned medium from the human MC line HMC-1 (HMC-1 CM) to examine its effect on macrophage polarization. HMC-1 CM was introduced at various stages: during the initiation of polarization into M1 and M2 phenotypes, after polarization, and to undifferentiated M0 macrophages. Additionally, we investigated the role of MC-derived tryptase in macrophage polarization using a tryptase inhibitor. Our findings show that HMC-1 CM significantly stimulated M1 marker expression in M0 macrophages when applied at the onset of culture. Moreover, HMC-1 CM increased the expression of Tgfb and Il10 in both M0 and M1-like macrophages, an effect that was diminished by the tryptase inhibitor. These results suggest that tryptase derived from MCs plays a crucial role in mediating macrophage polarization.

Keywords
Mast cell
Macrophage polarization
Tryptase
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Information
  • Publisher :The Korean Society for Microbiology and The Korean Society of Virology
  • Publisher(Ko) :대한미생물학회‧대한바이러스학회
  • Journal Title :JOURNAL OF BACTERIOLOGY AND VIROLOGY
  • Volume : 55
  • No :1
  • Pages :79-90
  • Received Date : 2024-11-05
  • Revised Date : 2025-02-03
  • Accepted Date : 2025-02-05
  • DOI :https://doi.org/10.4167/jbv.2025.55.1.079
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