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2024 Vol.54, Issue 2 Preview Page

Original Article

Multi-Drug Resistant Bacillus cereus: A Growing Concern for Powdered Infant Formula and Baby Foods (Cereals) in Bangladesh
Nazia Afrin1
,
Md. Shakhawate Hossain2*
,
RashedaYasmin Shilpi1
1Department of Botany, Jahangirnagar University, Dhaka-1342, Bangladesh
2Department of Fisheries Biology and Aquatic Environment, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur-1706, Bangladesh
*shakhawate@bsmrau.edu.bd
30 June 2024. pp. 122-133
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Abstract

Infants predominantly consume dried powdered infant formula (PIF) and cereals, which are highly susceptible to contamination by Bacillus cereus, the most prevalent pathogenic and toxin-producing bacterium. To investigate the microbial risk associated with Bacillus cereus, six samples of PIF and four cereals were procured from commercial markets in Bangladesh for analysis of bacterial prevalence. A total of twenty-eight bacterial isolates were obtained using the selective agar culture method. Morphological and biochemical tests confirmed that twelve isolates (43%) were positive for B. cereus. Further confirmation was achieved through PCR amplification and sequencing of the 16S rRNA gene, demonstrating similarity in identification among three B. cereus isolates. Antibiotic sensitivity testing revealed that the confirmed isolates were susceptible to Kanamycin, Gentamycin, Amikacin, Tigecycline, and Ciprofloxacin while exhibiting resistance to Amoxicillin, Aztreonam, Penicillin-G, Cefixime, Cefotaxime, Cefepime, Ceftriaxone, Meropenem, Cotrimoxazole, and Ceftazidime. Consequently, implementing effective control measures in the production and handling processes of PIF and other baby food products is imperative to mitigate the contamination risk posed by the endospore-forming and heat-resistant B. cereus.

Keywords
Occurrence
Antibiotic sensitivity
Resistant bacteria
Bacillus spp.
Powdered infant formula
16S rRNA gene
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Information
  • Publisher :The Korean Society for Microbiology and The Korean Society of Virology
  • Publisher(Ko) :대한미생물학회‧대한바이러스학회
  • Journal Title :JOURNAL OF BACTERIOLOGY AND VIROLOGY
  • Volume : 54
  • No :2
  • Pages :122-133
  • Received Date : 2024-05-04
  • Revised Date : 2024-06-10
  • Accepted Date : 2024-06-11
  • DOI :https://doi.org/10.4167/jbv.2024.54.2.122
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